Part:BBa_K2382009:Design

Thioredoxin-MSMEG_5998 fusion protein

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 367
  Illegal XhoI site found at 373
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 812
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

The usage of thioredoxin is to make MSMEG_5998 more soluble. However we don't want the two small protein to interrupt the folding of each other. That's why we add some linkers between them.

Source

thioredoxin

Escherichia coli str. K-12 substr. MG1655 ( NC_000913.3 )

MSMEG_5998

Mycobacterium smegmatis str. MC2 155 ( NC_008596.1 )